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KMID : 0603520040090020092
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Journal of Korean Association of Cancer Prevention
2004 Volume.9 No. 2 p.92 ~ p.97
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Effect of Rice Hull Extracts on Oxidative DNA Damage in Human Lymphocytes
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Jeon Kyung-Im
Park Hae-Ryong
Lee Seung-Cheol
Park Eun-Ju
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Abstract
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Rice hull is known to contain an antioxidant defense system to protect rice seed from oxidative stress. The aim of this study was to evaluate the effect of methanol extract of rice hull on oxidative DNA damage induced by H2O2 in human lymphocytes. Cells incubated in medium with 1 % DMSO (negative control) or with various concentration of rice hull extracts (12.5, 25, 50, 100μg/ml) for 30 min at 37oC were further treated with H2O2 (200μM) as an oxidative stimulus for 5 min on ice. Oxidative damage was evaluated by Comet assay (single-cell gel electrophoresis) and quantified as % tail DNA. Cell viability measured by trypan blue exclusion test was above 95% for all treatments. An increased oxidative DNA damage by H2O2 expressed by % tail DNA was significantly inhibited by pre-incubating with rice hull extracts in a dose dependent manner. When human lymphocytes were post-incubated with rice hull extract for 30 min after exposure to H2O2 or incubated with rice hull and H2O2 simultaneously, the protective ability of the rice hull was not changed. These results indicate that rice hull supplementation to human lymphocytes followed by H2O2 treatment inhibited damage to cellular DNA, supporting a protective effect of rice hull against oxidative damage.
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KEYWORD
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Rice hull extract, DNA damage, Comet assay, Hydrogen peroxide, Lymphocytes
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